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M5 Anti-HA Tag Rabbit Polyclonal Antibody 抗HA标签兔多克隆抗体
货号 规格 销售价
MF084-R-T 20ul 600.00
MF084-R-01 100ul 1500.00
  • 说明书:

    MF084-R M5 Anti-HA Tag Rabbit Polyclonal Antibody 抗HA标签兔多克隆抗体.pdf

     

     

    STORAGE

    Store at -20°C. Stable for one year from the date of shipment.

     

     

    BACKGROUND

    Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA). The HA epitope tag is useful in Western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-epitope tag.

     

     

    REFERENCES

    1. Hopp, T.P., Prickett, K.S., Price, V.L., Libby, R.T., March, C.J., Cerretti, D.P.,Urdal, D.L. and Conlon, P.J. 1988. A short polypeptide marker sequence useful for recombinant protein identification and purification. Nat. Biotechnol. 6: 1204-1210.

    2. Smith, D.B. and Johnson, K.S. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with Glutathione S-transferase. Gene 67: 31-40.

     

     

    SOURCE

    This monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the infuenza hemagglutinin epitope (YPYDVPDYA) (KLH-coupled).

     

     

    SPECIFICITY

    HA-Tag (26D11) Mouse mAb detects over-expressed proteins containing the HA epitope tag.

     

     

    REACTIVITY

    All

     

     

    ISOTYPE

    Mouse IgG1

     

     

    IMPORTANT

    Use an anti-MOUSE secondary antibody to detect the 26D11 antibody.

     

     

    RECOMMENDED ANTIBODY DILUTIONS

     

    Western blotting            1:5000

    Immunofluorescence        1:1000

    Immunoprecipitation         1:100

    ELISA                     1:2000

     

    For Western blots, incubate membrane with diluted antibody in 5w/v nonfat dry milk,1X TBS,0.05Tween-20 at 4˚C with gentle shaking,overnight.